Figure 1. Immunofluorescence assay of B3B9 and N297Q-B3B9 rIgG against four DENV serotypes.

Vero cells were infected with DENV1, 2, 3, or 4 at MOI 0.1. The ability of the HuMAbs B3B9 (A–D) and N297Q-B3B9 rIgG (F–I) in the culture supernatants of HEK293T cells transiently expressing these antibodies to bind to different serotypes of DENV was assessed by performing IFAs. (E, J) Negative control of mock infected cells. Representative images are shown.