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. 2017 Nov 13;5:e4021. doi: 10.7717/peerj.4021

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©2017 Injampa et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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NT levels of N297Q-B3B9 rIgG and B3B9 rIgG of DENV1-4 (A–D) in Vero cells were assessed by foci reduction neutralization tests. The foci of infected cells were counted and compared with the no antibody control, and the results were calculated as the percent reduction in focus forming units. The number of foci was calculated as the average of triplicate experiments. (The error bars show standard deviation of the experiments). (E) NT50 concentration (concentration that was used to reduce half of infected cells when compared with control) of N297Q-B3B9 and B3B9 rIgG antibody against DENV serotypes.