FIG. 4.

Dependence of EV uptake on cell growth matrix stiffness. (A) Illustration of experimental design. (B) Gelatin matrix stiffness quantified by rheology (no cells added). (C) CD63-GFP-labeled EV uptake by MCF-7 cells or MDA-MB-231 cells during culture on various substrates following 2 h of incubation. Experiments were performed in biological triplicate, and error bars indicate one standard deviation. Statistical tests comprise paired two-tailed Student's t-tests, individually comparing each gelatin concentration condition to TCPS (*p < 0.05). Results are representative of two independent experiments (Supplementary Fig. S8). TCPS, tissue culture polystyrene. Color images available online at www.liebertpub.com/tea