Table 4.
Method validation parameters for five reference analytes
| Parameters | Analytes | ||||
|---|---|---|---|---|---|
| Catechin | Kaempferol | Ferulic acid | Gallic acid | Paclitaxel | |
| Regression equation | 6.01× + 0.33 | 5.53× + 0.31 | 40.02× + 0.05 | 41.44× + 0.02 | 306× + 1.92 |
| Correlation coefficient (r2) | 0.9998 | 0.9999 | 0.9995 | 1.0000 | 0.9996 |
| Linearity range (ng/mL) | 1–250 | 1–250 | 0.5–100 | 0.1–100 | 5–500 |
| LOD (ng/mL) | 0.14 | 0.20 | 0.03 | 0.01 | 0.02 |
| LOQ (ng/mL) | 0.43 | 0.61 | 0.09 | 0.03 | 0.07 |
| Precision RSD % (Intra-day, n = 6) | 0.34 | 1.02 | 0.62 | 0.25 | 0.61 |
| Precision RSD % (Inter-day, n = 6) | 1.01 | 1.22 | 1.11 | 0.94 | 1.21 |
| Stability RSD % (n = 5) | 1.83 | 2.55 | 1.88 | 1.92 | 1.80 |
| Recovery (n = 3) Mean | 105.19 | 97.50 | 94.87 | 95.50 | 98.86 |
| RSD % | 0.76 | 1.02 | 1.13 | 1.82 | 0.96 |