Skip to main content
. Author manuscript; available in PMC: 2017 Nov 16.
Published in final edited form as: Biochem J. 2015 May 1;467(3):461–472. doi: 10.1042/BJ20141142

Figure 8.

Figure 8

Phenformin and PT-1 are equally effective in stimulating the activity of AMPK complexes containing γ1, γ2 and γ3 in HEK-293 cells. (A) Characterization by Western blotting of parental HEK-293 cells and cells stably expressing either wild type -γ1, -γ2 or -γ1. Duplicate cell lysates were blotted with the indicated antibodies. (B) Bar chart showing AMPK activity measured in anti-FLAG immunoprecipitates of cells treated for 1 hr with phenformin (10 mM) or PT-1 (100 μM). Results are mean ± SD (n = 3, separate cell incubations), and results that are significantly different from the relevant control without phenformin or PT-1 (1-way ANOVA with Sidak’s multiple comparison test of selected datasets) are shown with asterisks (**** p<0.0001). The lower panels show Western blots of duplicate dishes of cells probed using anti-pT172 (top), anti-AMPK-α (centre) or anti-FLAG (bottom).