Skip to main content
. Author manuscript; available in PMC: 2018 Nov 2.
Published in final edited form as: Cell Stem Cell. 2017 Nov 2;21(5):604–617.e5. doi: 10.1016/j.stem.2017.10.003

Figure 3. Activity of VGATMS-DG projections bi-directionally regulate quiescence/activation of NSCs.

Figure 3

(A) AAV-DIO-ChR2-YFP injection scheme to the MS and projections from MS to DG in the VGAT-Cre mice. (B) Trace showing dentate PV cells reliably follow a 10 Hz train of 1 ms, 470 nm light stimulation of VGATMS-DG projections without synaptic failures. Synaptic responses were blocked by bicuculline. (C) Experimental paradigm for in vivo optogenetic stimulation. (D) Confocal images showing quiescent (nestin+EdU−) and activated (nestin+EdU+) rNSCs. Scale bar: 50 µm (left), 10 µm (right zoomed-in). (E–G) Density of activated rNSC (nestin+EdU+), proliferating cells (EdU+), and the rNSC pool (nestin+). (n=8 for control, and n=6 for ChR2). (H) AAV-DIO-hM4Di-mCherry injection scheme to the MS and projections from MS to DG in the VGAT-Cre mice. (I) Experimental paradigm for in vivo CNO infusion. (J–L) Densities of activated rNSC (nestin+EdU+), proliferating progenitors and neuroblasts (EdU+), and rNSC pool (nestin+). (n=3 for control, and n=5 for hM4Di). *p<0.05 by Student’s t-test. Values represent mean ± S.E.M. See also Figure S3.