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. 2017 Sep 15;8(49):84697–84713. doi: 10.18632/oncotarget.20949

Figure 2. Varied effects of MEKi trametinib on various BRAF gene fusions.

Figure 2

A. GNAI1-BRAF, MACF1-BRAF, MKRN1-BRAF, FXR1-BRAF, or CLCN6-BRAF expressing NIH3T3 cells were serum starved for 24 hours and then incubated with increasing concentrations of drug for 1 hour. Corresponding cell lysates were immuno-blotted with indicated antibodies. B. BRAF-fusion expressing NIH3T3 were assayed for colony formation in soft agar and anchorage independent growth in the presence of increasing concentrations of drug. Colony number quantification as before (with SEM of > 3 three different images); * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.