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. 2017 Jun 21;8(49):84986–84995. doi: 10.18632/oncotarget.18583

Figure 2. Down-regulation of anti-apoptotic genes and MYC expression in response to combined CDK9 and BRD4 inhibition.

Figure 2

Rhabdoid tumor cell lines were treated for 120 min with increasing concentrations of BRD4 or CDK9 inhibitors alone or in combination. Expression of anti-apoptotic genes was analyzed by RT-qPCR. A significant down-regulation of anti-apoptotic genes is evident after exposure to JQ1/ LDC067 (A) or iBET/DRB (B). RT-qPCR analysis of de novo transcription was performed to assess if simultaneous treatment of RT cell lines can affect newly synthesized MYC mRNA using exon-exon primers (MYCe2e3) or intron-intron primers (MYCi1i1) after treatment with JQ1/ LDC067 (C) or iBET/DRB (D). Despite repressed MYC mRNA production, protein levels remain stable 24h after treatment of G401 cells with iBET or with DRB at low doses. Combination of both compounds synergistically reduces MYC protein levels (E). *p<0.05, **p < 0.01, ***p < 0.001 (ANOVA One-way Test).