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. 2017 Jun 21;8(49):84986–84995. doi: 10.18632/oncotarget.18583

Figure 3. Combined BRD4 and CDK9 targeting acts synergistically on inhibition of general transcription.

Figure 3

To detect effects of combined treatment strategy on general transcription, the indicated RT cell lines were incubated for 120 min with different concentrations of JQ1/ LDC067 (A, B) or iBET/DRB (C, D) alone or in combination of both inhibitors. Gene expression was analyzed by RT-qPCR using different exon-intron and exon-exon primer pairs. Newly synthesized mRNA of the housekeeping genes RPL3 (A, C) and GAPDH (B, D) is shown. Simultaneous application of the inhibitors significantly reduces the expression of these genes indicating a general blockade of the transcription process. *p<0.05, **p < 0.01, ***p < 0.001 (ANOVA One-way Test).