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. 2017 Jun 28;8(49):85024–85039. doi: 10.18632/oncotarget.18781

Figure 5. Breast tumour cancer cells express IFNγ inducing an increase in IDO1 expression in ECs.

Figure 5

(A) IFNγ mRNA expression is significantly up-regulated in MDA-MB-231 than those in MCF-10A (normal breast epithelial cells), which is further enhanced by low glucose (LGM) exposure for 48 hours compared with normal glucose (NM) medium. Also, IFNγ expression in MDA-MB-231 is reduced after co-cultured with endothelial cells for 48 hours. (B) Differential IDO1 mRNA expression in MDA-MB-231 and ECs occurs after single culture or co-culture for 48 hours. IDO1 mRNA levels significantly increase in endothelial cells after treated with IFNγ for 48 hours. (C) Western blot analysis confirms that IFNγ treatment induces an increase in IDO1 protein levels in endothelial cells using anti-IDO1 antibody (the upper panel), analysed by measuring band density (the low panel). GAPDH was used as an internal control.