Figure 2. Knockdown of LSD1 upregulates CD11b and CD86 expression in AML cell lines.
(A) RT-PCR analysis of CD11b and CD86 mRNA levels upon LSD1 knockdown in THP-1, Molm13 and Jurkat. (B) FACS analysis of CD11b surface expression levels upon LSD1 knockdown in THP-1, Molm13 and Jurkat. (C) FACS analysis of CD86 surface expression levels upon LSD1 knockdown in THP-1, Molm13 and Jurkat. 7-AAD and fixable viability dye as the indicator of cell viability.