Table 1. All primers used in this study are listed.
Gene symbol | Primer sequence | Tm (°C) |
---|---|---|
wtMkrn3 | F: 5′-ATGGAAGAGTCTACAGCTCCCATTG-3′ | 58 |
R: 5′-CTACAGATTCAAACTGAAATATTCTTCC-3′ | ||
mtMkrn3 (347-401 deletion) |
F: 5′-CGCAGCATGGACAAAGTGGTCTCCTCTGGCTTTGTC-3′ | 50 |
R: 5′-GACAAAGCCAGAGGAGACCACTTTGTCCATGCTGCG-3′ | ||
wtNptx1 | F: 5′-ATGCTGGCCGGCCGC-3′ | 63 |
R: 5′-TCAGTTGATCTGGCGACAAGCCTC-3′ | ||
mtNptx1 (268-302 deletion) |
F: 5′-TGCGGCGCCTGGAGTGCTGCCGTTTGTAATCAACG-3′ | 52 |
R: 5′-CGTTGATTACAAACGGCAGCACTCCAGGCGCCGCA-3′ |