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. 2017 Jul 7;8(49):85234–85251. doi: 10.18632/oncotarget.19076

Figure 7. MYCN and ALK copy numbers assessed in cell-free DNA from plasma reflect copy numbers determined from xenograft tumor genomic DNA.

Figure 7

(A) Schematic workflow summarizing xenografting of the OHC-NB1 neuroblastoma cell line and sample preparation from genomic DNA (gDNA) and cell-free DNA (cfDNA) for droplet digital PCR (ddPCR). OHC-NB1 cells were subcutaneously injected into both flanks of 3 NMRI-Foxn1nu mice for gene copy number determination. Genomic DNA was extracted from each xenograft tumor and fragmented by sonication prior to ddPCR. Total blood plasma from the 3 mice was pooled for cfDNA isolation. (B) White bars show MYCN and ALK copy numbers detected by ddPCR using xenograft tumor genomic DNA (mean ± SD; n = 6) as the reference base. MYCN (green bar) and ALK (blue bar) copy numbers detected by ddPCR of cell-free DNA isolated from pooled mouse plasma (n = 1) are shown.