Table 2. MYCN and ALK copy numbers determined by ddPCR from neuroblastoma patient blood plasma samples and paired genomic DNA from the corresponding primary tumor biopsy.
| MYCN copy number | ALK copy number | |||||
|---|---|---|---|---|---|---|
| Patient | genomic DNA (tumor biopsy) |
cell-free DNA (plasma) |
reference valuea (tumor biopsy) |
genomic DNA (tumor biopsy) |
cell-free DNA (plasma) |
reference valueb (tumor biopsy) |
| 1 | 1.70 | 7.30 | diploid | 2.09 | 2.30 | - |
| 2 | 1.73 | 2.65 | diploid | 2.10 | 2.00 | diploid |
| 3 | 1.93 | 2.60 | diploid | 2.09 | 1.88 | diploid |
| 4 | 2.14 | 5.10 | diploid | 2.20 | 2.10 | - |
| 5 | 2.16 | 2.70 | diploid | 2.02 | 2.80 | diploid |
| 6 | 85.0 | 60.5 | 20x amplified | 2.00 | 2.19 | diploid |
| 7 | 129.0 | 37.2 | 20x amplified | 2.19 | 3.20 | - |
| 8 | 73.5 | 13.5 | 30x amplified | 2.13 | 3.50 | - |
| 9 | 207.5 | 49.2 | 50x amplified | 3.40 | 3.07 | - |
| 10 | 267.0 | 83.0 | 50x amplified | 2.04 | 2.13 | - |
aFISH
bFISH or qPCR