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. 2017 Jul 28;8(49):85252–85262. doi: 10.18632/oncotarget.19654

Figure 1. The effects of F2 on HIF-1α and HIF-1β expression in U87 cells.

Figure 1

(A, B) Western blot analyses of HIF-1α and HIF-1β were done using total proteins extracted from U87 cells incubated under normoxic or hypoxic conditions for 16 h in the presence or absence of the indicated concentrations of F2. Values are means ± SEM of three experiments. ### P<0.0001 compared with the normoxic group; * P<0.05, **P<0.01 and ***P<0.001 compared with the group untreated with F2. (C) U87 cells were treated with F2 (30 μg/ml) for 8 h, 16 h and 24 h under normoxic and hypoxic conditions, and total proteins were collected for western blot analysis. Values are means ± SEM of three experiments. * P<0.05, **P<0.01 compared with normoxic group. $$P<0.01 and $$$P<0.001 compared with the group untreated with F2. (D) HIF-1α DNA binding activity assay was done using nuclear proteins extracted from U87 cells incubated under normoxic or hypoxic conditions for 16 h in the presence or absence of the indicated concentrations of F2. Values are means ± SEM of three experiments. ## P<0.01 compared with the normoxic group; ** P<0.01 compared with the hyooxic group on time point 0.