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. 2017 Jul 28;8(49):85252–85262. doi: 10.18632/oncotarget.19654

Figure 2. The effects of F2 on HIF-1α half-life, degradation and transcription.

Figure 2

(A) Cells were treated with DFX (100 μM) in the presence or absence of F2 (10 μg/ml) for 4 h, and then CHX (40 μg/ml) was added for the indicated time. Values were normalized to β-actin and expressed as a percentage relative to time 0, which was considered equal to 100 %. (B) U87 cells were treated with MG132 (20 μM), DMOG (100 μM) or DFX (100 μM) for 8 h under normoxic conditions in the presence or absence of the indicated concentrations of F2, then total proteins were collected for western blotting. (C) Real-time PCR analysis of HIF-1α mRNA using total RNA extracted from U87 cells incubated under hypoxia for 16 h in the presence or absence of the indicated concentrations of F2.