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. 2017 Jul 28;8(49):85252–85262. doi: 10.18632/oncotarget.19654

Figure 3. The effects of F2 on the EGFR/PI3K/AKT/mTOR and MAPK pathways.

Figure 3

(A, B) Effects of F2 on hypoxia-induced signaling pathways related to the expression of HIF-1α in U87 cells. Cells were pretreated with the indicated concentrations of F2 for 1 h, then incubated under normoxic and hypoxic conditions for 1 h. Total proteins were then collected for western blotting using antibodies as indicated. (C) Effects of inhibitors on hypoxia-induced expression of HIF-1α in U87 cells. Cells were pretreated with LY (LY294002, 100 μM), Rap (Rapamycin, 100 nM), PD (PD98059, 100 μM) and AG1478 for 1 h, and then incubated under normoxic or hypoxic conditions for 16 h. Total proteins were subjected to western blotting using antibodies against HIF-1α. (D) Cells were pretreated with or without LY (LY294002, 100 μM) or PD (PD98059, 100 μM), then co-treated with or without F2 (10 μg/ml) for 16 h under hypoxic conditions. LY’ and PD’ indicate cells treated with inhibitors and F2. Total proteins were collected for western blot analysis.