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. 2017 Jul 28;8(49):85252–85262. doi: 10.18632/oncotarget.19654

Figure 5. The effects of F2 on VEGF expression and formation of tubular structures by endothelial cells.

Figure 5

(A, B) VEGF protein expression was evaluated by western blot analysis of protein extracts from U87 cells (A, upper panel) and ELISA in the culture supernatant (B, right panel) after incubation under hypoxia in the presence or absence of the indicated concentrations of F2. RT-PCR (A, lower panel) and real-time PCR analysis (B, left panel) of VEGF mRNA were carried out using total RNA prepared from U87 cells incubated under hypoxia for 16 h in the presence or absence of the indicated concentrations of F2. (C) Western blot analysis of HIF-1α and VEGF was carried out using total proteins from U87 cells transfected with HIF-1α siRNA or control siRNA and treated under normoxic and hypoxic conditions for 16 h. (D) The tube formation assay was performed as described in the Methods section. Values are means ± SEM of three experiments. ### P<0.01 compared with the normoxic group; ** P<0.01 compared with the hypoxic group.