Figure 2. Expression of IL-31, TSLP and their receptors in lymphoid cells populating HL and reactive lymph node microenvironment.

Left panels. A representative gating strategy for B cells (CD19⁺), T cells (CD4⁺), and macrophages (CD68⁺). Middle panels. IL-31/TSLP expression was analyzed by flow cytometry at surface and intracellular levels on CD19⁺, CD4⁺, and CD68⁺ cells for both HL and reactive lymph nodes. Results are shown in box plot as median MRFI, first and third quartiles, maximum and minimum values, from 7 different HL and 7 reactive lymph node cell suspensions. Right panels. IL-31RA/OSMR and TSLPR/CD127 chain receptor expression was analyzed by flow cytometry on CD19⁺, CD4⁺ and CD68⁺ cells for both HL and reactive lymph nodes. Results are shown in box plot as median MRFI, first and third quartiles, maximum and minimum values, from 7 different HL and 7 reactive lymph node cell suspensions.