Figure 3. Effect of miR-21 and EZH2 on colony-formation, migration and invasion of LCSCs.

(A) Colony-formation assays was decreased significantly after EZH2 or miR-21 knockdown, but rescued the effects of anti-miR-21 after overexpression of exogenous EZH2 in LCSCs; (B) & (C) cell migration and invasion were reduced significantly by EZH2 or miR-21 knock down, but exogenous EZH2 over expression rescued the effects of anti-miR-21 in LCSCs. Each experiment was performed in triplicate, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05.