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. 2017 Sep 14;8(49):85492–85503. doi: 10.18632/oncotarget.20888

Figure 1. hTERT expression in squamous cell carcinoma tissues and cell lines.

Figure 1

(A) The relative protein expression of hTERT was detected by western-blotting analysis in paired clinical squamous cell carcinoma tissue (T) and adjacent non-tumor tissues (N), with each pair obtained from the same patient. (B) RT-PCR analysis of expression of hTERT in different oral squamous cell lines (normal human epithelial (NHE) cells, SCC-15, OSC-19, Cal-27 and 8113). The results are representative of at least 3 replicates. (C) HE (1, 2) and immunohistochemical staining (3–6) of paraffin-embedded sections in control group (upper panel) and tongue carcinoma (lower panel) were performed using an antibody against TERT using the streptavidin peroxidase (SP) method. Images 1 and 2, 100 ×magnification, images 3 and 4, 200× magnification, and images 5 and 6, boxed areas in 3 and 4, 400× magnification.