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. 2017 Sep 14;8(49):85492–85503. doi: 10.18632/oncotarget.20888

Figure 5. Knock-out hTERT inhibits EMT in SCC-15 cells.

Figure 5

(A) Morphological changes in cells with hTERT knockout compared to control cells. Knockout of hTERT resulted in greater cell-cell contacts when compared with scramble control cells. (B) mRNA levels of several EMT makers in scramble and the hTERT−/− cells (n = 3). *P < 0.05; **P < 0.01. (C) Protein levels of EMT markers, E-cadherin and SNAIL in SCC-15 stable cells assessed by western blotting. (D) Fluorescence microscopic staining of e-cadherin (green) is indicated in scramble and knockout cells. Nuclear DNA was stained with DAPI (blue). Scale bar: 100 μm. Data were collected in this set of figures from a representative of at least 3 independent experiments. (E) Immunofluorescent staining of F-actin using phalloidin–fluorescein isothiocyanate in scramble and the hTERT−/− cells.