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. 2017 Aug 18;8(49):85584–85598. doi: 10.18632/oncotarget.20337

Figure 6. HCV Core overcomes ATRA-induced apoptosis during virus infection.

Figure 6

Huh7.5 cells were either mock-infected or infected with the JFH-1 strain of HCV at an MOI of 1.0 for 48 h in the presence or absence of 5 μM ATRA. (A) Nuclei (blue) and HCV Core (green) in the mock-infected and HCV-infected Huh7.5 cells were detected by DAPI staining and immunofluorescence assay, respectively. (B) Levels of the indicated proteins were measured by western blotting. (C) DNMT activity was determined as described in the Methods section (n = 4). (D) Genomic DNA isolated from cells was subjected to MSP analysis to determine the methylation status of the p14 promoter. For lane 5, the cells were treated with 5 μM 5-Aza-2′dC for 24 h before harvesting. (E) Cells were treated with 5-Aza-2′dC as described in Figure 4C and levels of the indicated proteins were measured by western blotting. (F) The graph shows the percentage of TUNEL-positive cells prepared as in (G) (n = 4). (G) Upper panels show total nuclei (blue) detected by DAPI staining and lower panels show TUNEL-positive nuclei (green) in Huh7.5 cells prepared as in (E).