Table 1. PCR primer sequences.

Primer	Sequence (5′-3′)
Quantitative reverse-transcription PCR
Forward primer	CAACTCAGGGCACTGTGACC
Reverse primer	GGCTCTGCCCACATTGCTTC
Methylation-specific PCR
Methylated forward primer	TAAGTTATTTTTTATCGTTTAGCGT
Methylated reverse primer	TAATATAATTTTCATAAATCCCGAA
Unmethylated forward primer	GGATAAGTTATTTTTTATTGTTTAGTGT
Unmethylated reverse primer	TAATATAATTTTCATAAATCCCAAA
Sequenom MassARRAY PCR
Forward primer	TTGAATGGTTTTATGGAATGATGTA
Reverse primer	CACACCTCAAAAAAACTAAAATAACC