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. 2017 Aug 24;8(49):85759–85771. doi: 10.18632/oncotarget.20482

Figure 4. Comparison of the larger ERCC1 expression by cisplatin in different ovarian cells.

Figure 4

(A) Ovarian normal cells ISOE80, ovarian cancer cells PEO14 and A2780 were untreated, or treated with cisplatin (6 μM) for 48 h. Histogram shows the mean level (± SD) of larger ERCC1 mRNA relative to β-Actin determined from three independent experiments by qRT-PCR. Data were normalized to the mean level of larger ERCC1 relative to β-Actin in the controls. (B) Histogram shows the ratio (± SD) of larger ERCC1 mRNA relative to total ERCC1 mRNA determined from three independent experiments by qRT-PCR. Larger ERCC1 mRNA and total ERCC1 mRNA levels were each calculated relative to β-Actin. (C) Platinum-sensitive ovarian cancer cells A2780 and platinum-resistant A2780/DDP were untreated, or treated with cisplatin (6 μM) for 48 h. Histogram shows the mean level (+ SD) of larger ERCC1 mRNA relative to β-Actin determined from three independent experiments by qRT-PCR. Data were normalized to the mean level of larger ERCC1 relative to β-Actin in the A2780 control. Student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.