Table 2. All primers used for 5’ RACE assay.
Name | Sequence 5'-3' | Description |
---|---|---|
H-exon 2-3 reverse | ATTTGTGATACCCCTCGACGAG | Normal human ERCC1 transcript reverse primer |
Hskin4RT reverse |
TGTACAGAGATCGCCCTGCTCTATGC | Larger Human ERCC1 transcript reverse primer |
REV1 | CGGTCCTCCAGAACCATAGA | Larger Human ERCC1 transcript reverse primer |
Oligo dT anchor | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV (V=A, C or G) | Anchor primer |
PCR anchor | GACCACGCGTATCGATGTCGA | Internal anchor primer |