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. 2017 Aug 24;8(49):85858–85867. doi: 10.18632/oncotarget.20691

Figure 2. The role of MYC in glutaminolysis and glycolysis in HMCLs.

Figure 2

(a) Western Blot shows protein expression in U266 cells before and after lentiviral MYC-transduction (U266/U266-MYC) compared to endogenous expression levels in INA-6. MYC expression significantly elevates GAC and ASCT2 protein expression in U266 cells. (b) Oligomycin and 2-deoxyglucose (2-DG) treatment of INA-6, U266 and U266-MYC. Comparison of ATP-generation from glycolysis (2-DG incubation) and oxidative phosphorylation (oligomycin treatment) revealed that INA-6 cells synthesize ATP via glycolysis independent of oxidative phosphorylation as 2-DG reduces measurable ATP. In contrast, U266 produce ATP via mitochondrial respiration, which can be blocked using the ATP-synthase inhibitor oligomycin. INA-6 and U266 cells use contrary mechanisms to support ATP generation. U266-MYC cells are more sensitive to glycolysis inhibition and less sensitive to inhibition of the oxidative phosphorylation pathway (n=3). (c) Western Blot of MM cell lines incubated with or without glucose and L-glutamine in the culture medium for 24 hours. All HMCLs tested showed minimal changes in both MYC expression and generation of cleaved active caspase 3 when glucose is removed from the media. Removal of glutamine however diminishes MYC expression and increases cleaved active caspase 3 levels. For U266-MYC both withdrawal conditions reduce MYC protein while caspase 3 is cleaved.