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. 2017 Sep 15;8(49):86031–86042. doi: 10.18632/oncotarget.20907

Figure 2. MPLA induced translocation of NF-kB p65 and activated MAPK signaling pathway.

Figure 2

At 0.5h and 2h after 8Gy irradiation, HUVEC cells were stained with NF-kB p65 antibody (A) and p65 fluorescence inside and outside the nuclear were quantified and the ratio was calculated (B). NF-kB luciferase activity was measured in 293T cells after different centration of MPLA treatment (C). Western blot assay of TRIF, MyD88, and MAPK signaling pathway were examined at 0, 0.5 and 8h after irradiation (D). Raw density of TRIF (E), MyD88 (F), p-p38 (G) and pJNK (H), compared to internal control, was analyzed. **P<0.01 Vs IR groups. (n=8)