Table 1. Primer pairs in the study.
Primer | Sequence (5′ to 3′) | Nucleotide position (nt) | Remark |
---|---|---|---|
UCHL1-F | CCAAAGAGCACGCAGACATGC | 179-299 | UCHL1 fragment |
UCHL1-R | TTAAGACTTGCTGAGCGCCAC | 844-864 | |
AP | GGCCACGCGTCGACTAGTACT18 | 3′ Race | |
AAP | GGCCACGCGTCGACTACGGGIIGGGIIGGGIIG | 5′Race | |
AUAP | GGCCACGCGTCGACTAGTAC | 3′, 5′Race | |
5′RTP | GGCATTCGTCCATCCAGCTC | 715-734 | 5′Race |
5′GSP1 | GGCATTCGTCCATCCAGCTC | 715-734 | 5′Race |
5′GSP2 | GTTCACTTTGTCATCCACCC | 653-672 | 5′Race |
5′GSP3 | CTTTGTCATCCACCCGGCAG | 658-677 | 5′Race |
3′GSP1 | CCCTCTCACTCCTCAGCATG | 354-373 | 3′Race |
3′GSP2 | CTGTGCTGTTCTTCTGCTCT | 333-352 | 3′Race |
3′GSP3 | GGATGTGCTTGAGTTCTCAG | 282-302 | 3′Race |
F-UCHL1 | GTGGTTAGAGGTCCAAAGAG | 167-186 | qPCR |
R-UCHL1 | ATCCACGAAGTGCCAACCAG | 266-285 | |
F-β-actin | AAAAGCCAACCGAGAAAAG | qPCR | |
R-β-actin | TACGACCAGAGGCATACAG |