Figure 1. Baicalein inhibits osteosarcoma cells proliferation in vitro.

(A) Molecular structure of baicalein. The molecular formula of baicalein is C₁₅H₁₀O₅ and its molecular weight is 270.24. (B and C) 143B and MG-63 cells were incubated with increasing doses of baicalein (20–200 μM) for 24, 48 and 72 h. CCK-8 assay was performed to determine the cytotoxic effect of baicalein. Each point represents means ± SD of three independent experiments. (D) Normal human osteoblast cells were incubated with increasing doses of baicalein (20–200 μM) for , 24, 48 and 72 h and analyzed for cell proliferation. Each point represents means ± SD of three separate experiments. (E) Representative images from the colony-formation assay. Osteosarcoma 143B cells were incubated with or without baicalein (60 and 120 μM) for 24 h and allowed to grow into colonies for 14 d. (F) Quantitative analysis of colony forming rate of 143B and MG-63 cells. Each bar represents means ± SD of three separate experiments. **p < 0.01, ***p < 0.001.