Figure 7. Baicalein inhibits osteosarcoma143B cell growth in vivo.
(A) The mice body weight (in grams, recorded every week) were recorded and compared. (B) Representative images of 143B xenograft vehicle and treatment with low and high dose of baicalein. (C) The tumor volume were recorded and compared (volume in mm3, recorded every week). (D) Tumor weight was obtained at the end of the experiment. (E) Survival rate of mice after the indicated treatment were presented. (F) Immunohistochemical (IHC) analysis of Ki-67 and TUNEL staining for tumors of all treatment groups ( × 400, magnification). (G) Quantitative analysis IHC and apoptotic cells as shown in F, each group were counted on the basis of viewing eight random fields in each glide. (H) The proteins expression of β-catenin, c-myc, cyclinD1, and survivin in xenograft tumor tissues of all treatment groups. GAPDH was used as a loading control. all data are expressed as the mean ± SD of three independent experiments. NS, not significant. *P<0.05, **P<0.01.