Table 2. Primer sequences used for real-time PCR analysis.
| Genes | Primer sequence | |
|---|---|---|
| Cyclin D1 | Forward Reverse |
5’-ACAAACAGATCATCCGCAAACAC-3’ 5’-TGTTGGGGCTCCTCAGGTTC-3’ |
| Cyclin E1 | Forward Reverse |
5’-CCACACCTGACAAAGAAGATGATGAC-3’ 5’-GAGCCTCTGGATGGTGCAATAAT-3’ |
| CDK4 | Forward Reverse |
5’-GAGGGGGCCTCTCTAGCTT-3’ 5’-CACGGGTGTAAGTGCCATCT-3’ |
| c-myc | Forward Reverse |
5’-GCCACGTCTCCACACATCAG-3’ 5’-TGGTGCATTTTCGGTTGTTG-3’ |
| Bax | Forward Reverse |
5’-GAAGCCGGTGGTGGAGAA-3’ 5’-GCTTGGAGTTGGGCTGGTG-3’ |
| Bcl-2 | Forward Reverse |
5’- GAAGCAGGTAATGGAGCAAGGA-3’ 5’-GAAGCGTAGTTGTTGAGATGCG-3’ |
| MMP-2 | Forward Reverse |
5’-GAGTGCATGAACCAACCAGC-3’ 5’-AAACTTGCAGGGCTGTCCTT-3’ |
| MMP-9 | Forward Reverse |
5’-TCTATGGTCCTCGCCCTGAA-3’ 5’-TTGTATCCGGCAAACTGGCT-3’ |
| Survivin | Forward Reverse |
5’-ATTTGAATCGCGGGACCC-3’ 5’-GAGAAAGGGCTGCCAGGC-3’ |
| GAPDH | Forward Reverse |
5’-ACTTTGGTATCGTGGAAGGACTCAT-3’ 5’-GTTTTTCTAGACGGCAGGTCAGG-3’ |