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. 2017 Sep 21;8(49):86240–86252. doi: 10.18632/oncotarget.21133

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Copyright: © 2017 Gao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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SPARC was detected by goat anti-human SPARC and Alexa Fluor 488-conjugated donkey anti-goat IgG secondary antibody (indicated in green). Tumor-associated macrophages were identified by mouse anti-human CD68 antibody and Cy3-conjugated donkey anti-mouse IgG secondary antibody (indicated in red). Nuclei were stained with DRAQ5 (indicated in blue), and yellow staining indicates the co-localization of SPARC protein (green) and CD68 (red). All pictures were taken at 63x magnification under an oil objective. The distribution of SPARC+/ CD68+ cells in moderately differentiated tubular adenocarcinoma, scale bar: 25 μm (A); in poorly differentiated adenocarcinoma, scale bar: 25 μm (B); in well differentiated papillary adenocarcinoma, scale bar: 25 μm (C); typical SPARC+/CD68+ cells in well differentiated papillary adenocarcinoma, scale bar: 2.5 μm (D).