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. 2017 Sep 21;8(49):86240–86252. doi: 10.18632/oncotarget.21133

Figure 5. The expression of SPARC protein in a co-culture model of human fibroblasts and gastric cancer cells.

Figure 5

(A) The expression of SPARC protein in human fibroblast cells (BJ) and gastric cancer cells (AGS, MKN-45) was visualized by immunofluorescent staining. SPARC was detected by goat anti-human SPARC and Alexa Fluor 488-conjugated donkey anti-goat IgG secondary antibody (indicated in green). Nuclei were stained with DRAQ5 (indicated in blue). All pictures of immunofluorescence staining were taken at 63x magnification under an oil objective, scale bar: 25 μm. (B) The expression of SPARC protein in human fibroblast cells (BJ) and gastric cancer cells (AGS, MKN-45) was visualized by western blotting. SPARC protein in human fibroblast cells (BJ) and gastric cancer cells (AGS, MKN-45) were demonstrated by probing the cell lysates with goat anti-human SPARC antibody (AF941, R&D system), molecular weight: 40kDa. GAPDH was tested in the same gel as internal control for the loading sample, molecular weight: 37kDa. The relative abundance of SPARC expression in different lanes was demonstrated via normalization with the expression of GAPDH. BJ, AGS and MKN-45 meant that the cells were cultured alone for 48h; BJ+AGS and BJ+MKN-45 meant that BJ cells were co-cultured with AGS cells or BJ+MKN-45 cells for 48h, respectively.