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. 2017 Sep 21;8(49):86312–86324. doi: 10.18632/oncotarget.21146

Figure 3.

Figure 3

(A) Dramatical growth inhibition of MYCN-amplified SCLC cells induced by co-treatment of JQ1 and ABT-263. H526 and H69 cells were treated with DMSO control, JQ1, ABT-263 or the combination of JQ1 and ABT-263 for 72 hours. After treatment, growth inhibition was determined by CellTiter-Glo Luminescent assay. (B) JQ1 and ABT-263 co-treatment dramatically induces apoptosis in MYCN-amplified SCLC cells. H526 and H69 cells were treated with DMSO control, JQ1, ABT-263, or the combination of JQ1 and ABT-263 for 24 hours. PARP cleavage was detected by western blotting. β-Actin was used as a loading control. (C) Knock-down of Bim is confirmed by western blotting. After H69 cells were transfected by Bim siRNA #1, Bim siRNA #2 and control siRNA for 48 hours, cellular proteins were collected, and then Bim was detected by western blotting. (D) Knockdown of Bim significantly decreases the growth inhibition of H69 cells treated with combination of JQ1 and ABT-263. H69 cells were transiently transfected with Bim siRNA #1, Bim siRNA #2 and control siRNA for 24 hours. Then, transfected cells were plated in 96-well plate, and incubated with DMSO, JQ1 (100 nM), ABT-263 (50 nM) or the combination of JQ1 (100 nM) and ABT-263 (50 nM) for 72 hours. Cell proliferation was evaluated by CellTiter-Glo Luminescent assay.