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. 2017 Sep 21;8(49):86312–86324. doi: 10.18632/oncotarget.21146

Figure 4. Combination treatment of JQ1 and ABT-263 strongly disrupts the interaction of Bim with Bcl-2 and Mcl-1.

Figure 4

H526 cells were treated with DMSO control, JQ1 (100 nM), ABT-263 (200 nM), JQ1 (100 nM) plus ABT-263 (200 nM) for 24 hours. After treatment, Cells were lysed in immunoprecipitation lysis buffer. Cellular extracts were immunoprecipitated with 1 μg anti-Bim antibody. Precipitates were analyzed by western blotting to detect Bim, Mcl-1 and Bcl-2 proteins. The total lysates were also subjected to western blotting to detect the expression of Bim, Mcl-1 and Bcl-2 proteins. β-Actin was used as a loading control. Protein band intensities were quantified by ImageJ.