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. 2017 Sep 23;8(49):86410–86422. doi: 10.18632/oncotarget.21195

Figure 5. The effect of the HOTAIR/miR-125 and miR-143/HK2 axis on cell proliferation, invasion and migration in ESCC Cells.

Figure 5

(A) Cell proliferation in KYSE30 cells was determined with CCK-8 assay. (B, C) Cell cycle distribution after transfecting hsa-miR-125a-5p mimics (B) or hsa-miR-143 mimics (C) into KYSE30 cells was determined by flow cytometry using propidium iodide (PI) staining. (D-I) The transmembrane migration and invasion capacity in KYSE30 cells (D, E, F) or KYSE180 cells (G, H, I) infected with hsa-miR-125a-5p or sh-NC. (J-O) The transmembrane migration and invasion capacity of KYSE30 cells (J, K, L) or KYSE180 cells (M, N, O) infected with hsa-miR-143 or sh-NC. (P, Q) Lactate production in the culture media of hsa-miR-125a-5p-transfected (P) or hsa-miR-143-transfected (Q) KYSE30 cells was detected using metabolic assays. Representative results from at least 3 independent experiments.