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. 2017 Sep 23;8(49):86576–86591. doi: 10.18632/oncotarget.21245

Figure 2. Antiviral activities of plasmids containing CpG ODNs in CIK cells.

Figure 2

(A) CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and subsequently challenged with GCRV at 48 h post plasmids administration. The mRNA expression levels of VP4 were examined at 0, 24 and 48 h post-challenge. The EF1α gene was used as an internal control to normalize the cDNA template. mRNA levels of the target gene were normalized to those in CIK cells at 0 h. The p value was calculated by student's t-test between experimental and control groups (*, p ≤ 0.05, **, p ≤ 0.01). Error bars indicate SE (n = 3). Blank columns for 24 h and shadowy for 48 h. (B) Crystal violet staining of CIK cells after plasmids containing CpG ODNs stimulation and GCRV infection at 48 h post plasmids stimulation. Control group was treated with PBS and subsequently GCRV while mock group without any treatment.