Skip to main content
. 2017 Sep 23;8(49):86576–86591. doi: 10.18632/oncotarget.21245

Figure 8. mRNA expressions of three immunoglobulins.

Figure 8

The mRNA expressions of CiIgM (A), CiIgD (B) and CiIgZ (C) were measured at D0, D1, D5, D10, D14, D16 and D20 post immunization or subsequent infection in spleen (Left) or head kidney (Right). Grass carp were vaccinated with normal saline (NS), vaccine, vaccine-pcCpG and pcCpG respectively, and spleen and head kidney tissues were obtained from 5 random grass carp from each group at each time point. Different columns represent different time points. The 18S rRNA gene was used as an internal control to normalize the cDNA template. mRNA levels of the target gene were normalized to those in spleen tissue at 0 h. Asterisks (*) mark the significant difference (P ≤ 0.05) between experimental and control groups and double asterisks (**) mark the extremely significant difference (P ≤ 0.01). Error bars indicate SE (n = 3).