Table 1. The sequences of primers used.
Gene | Sequence |
---|---|
miR-29a-3p | Forward primer: AGCACCAUCUGAAAUCGGUUA |
universal primer: GTGCAGGGTCCGAGGT | |
U6 | Forward primer: CTCGCTTCGGCAGCACA |
Reverse primer: AACGCTTCACGAATTTGCGT | |
IGF1R | Forward primer: CCCGGCATCTTACTACATGG |
Reverse primer: GAAGGAACTGAAGCATTGG | |
GAPDH | Sense: 5′-GGAGCCAAAAGGGTCATCATCTC-3′ |
Anti-sense: 5′-GAGGGGCCATCCACAGTCTTCT-3′ | |
CD8 | Sense: 5′-CGCTGTCAGATCCCCTTTGT-3′ |
Anti-sense: 5′-GAGGAAGGACCCTCTCCCTT-3′ | |
CCL5 | Sense: 5′-CAGTCGTCTTTGTCACCCGA-3′ |
Anti-sense: 5′-TGTAACTGCTGCTGTGTGGT-3′ |