Skip to main content
. 2017 Sep 30;8(49):86693–86709. doi: 10.18632/oncotarget.21411

Figure 2. Interaction of LNT with MCF-7 cells in vitro.

Figure 2

(A) Recognition and internalization of LNT by MCF-7 cells. After incubation at 4°C for 40 min (a) and at 37°C for 2 h (b) with FITC-LNT (200 μg/mL), MCF-7 cells were stained with Hoechst 33342, fixed and imaged by confocal microscopy using laser excitation at 405 and 488 nm. The micrographs were obtained at a magnification of 600 ×. (B, C) Cellular uptake of FITC-LNT quantitatively estimated by the fluorescence intensity of cell lysates treated with different concentrations at 4°C for 40 min and with FITC-LNT (200 μg/mL) at 37°C for different times. FITC-LNT was used as the reference. Values expressed are means ± standard deviation (SD) of triplicates. Scale bars, 25 μm.