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. 2017 Sep 30;8(49):86747–86768. doi: 10.18632/oncotarget.21421

Figure 1. Isolation of CD44v6 avid phage by affinity selection.

Figure 1

(A) The eluted phage titers of each round of affinity selection. The titers of fUSE5/15mer phage (white bar) or M13C7C phage (gray bar) were determined by measurement of virions per milliliter or plaque forming units per milliliter. (B) The selected frequency of eleven phage clones with unique sequences. These phage were chosen to test their binding to the v6 region peptide. (C) ELISA of the selected phage. After incubation with the immobilized v6 region peptide (white bar) or control peptide (gray bar), the bound phage were detected by a polyclonal HRP-conjugated anti-phage Ab. (D) ELISA of synthesized biotinylated peptides. After incubation with the immobilized v6 region peptide (white bar) or control peptide (gray bar), the bound peptides were detected by HRP-conjugated streptavidin.