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. 2017 Sep 30;8(49):86747–86768. doi: 10.18632/oncotarget.21421

Figure 3. PFT binding to PCa cell lines.

Figure 3

(A) Immunoblotting analysis of CD44v6 expression levels in three human PCa cell lines using the CD44v6-specific mAb VFF18. Lane 1, MDA-PCa-2b; Lane 2, PC3M; Lane 3, PC3. (B) A flow cytometry histogram plot of CD44v6 expression on the surface of the three PCa cell lines. The bound VFF18 (gray) or isotype control Ab (blank) were detected by APC conjugated secondary Ab. (C) Flow cytometry analysis of the binding of the four peptides (PFT, WAG, SAY and WSN) to the surface of the three PCa cell lines. The bound biotinylated peptides were detected by APC conjugated streptavidin. Bar graphs of quantitative MFI were used to compare the relative cell binding of the different peptides.