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. 2017 Sep 30;8(49):86747–86768. doi: 10.18632/oncotarget.21421

Figure 4. Establishment of v6A3 cell line.

Figure 4

(A) A schematic diagram of SORE6-GFP vector. In this vector, the GFP reporter was designed to be driven by six concatenated repeats of SOX2/OCT4 response element (SORE6) of the NANOG promoter. (B) The morphology of a v6A3 colony at 7 and 14 days. PC3M cells stably transfected with the SORE6-GFP construct were further treated with DOX. After being diluted and inoculated into 96 wells, one DOX resistant and GFP positive holoclone (green), v6A3, was used as an aggressive PCa cell line. Scale bar, 150 μm. (C) GFP positivity revealed by flow cytometry. The histogram plots represent bulk PC3M cells transiently transfected by maxGFP (∼91% GFP+) or SORE6-GFP (∼10% GFP+), and v6A3 cells (∼90% GFP+) after continuous passages for one month.