Skip to main content
. 2017 Sep 30;8(49):86769–86783. doi: 10.18632/oncotarget.21423

Figure 4. Reolysin decreases HIF expression and reduces VEGF secretion.

Figure 4

(A) Reolysin decreases HIF-1ɑ activity. HCT116 cells stably expressing a HIF-1ɑ luciferase reporter were treated with the indicated concentrations of Reolysin with or without the presence of 100 μM CoCl2 for 24 and 48 h. HIF-1ɑ activity was determined by measuring luminescence. Mean ± SD, n = 3, *Indicates a significant difference from Vehicle control cells, p < 0.05. (B) Reolysin decreases the expression of HIF-1ɑ, HIF-2ɑ, and GLUT1. Sarcoma cells were treated with 30 PFU/cell Reolysin with or without the presence of CoCl2 for 24 h and protein levels were measured by immunoblotting. Bands were quantified by densitometry. *Indicates a significant difference compared to Control or CoCl2 stimulated cells, p < 0.05. (C) Reolysin reduces VEGF secretion. HT-1080 and SK-LMS-1 cells were treated with 30 PFU/cell Reolysin for 6, 24, and 48 h with or without CoCl2. VEGF secretion was measured by ELISA. Mean ± SD, n = 3, *Indicates a significant difference from control cells at the same time, p < 0.05.