Figure 5. Reolysin augments the anticancer activity of temsirolimus, sunitinib, and bevacizumab.

(A) Anti-angiogenic agents increase Reolysin-induced CXCL10 levels. SK-LMS-1 cells were treated with 30 PFU/cell Reolysin, 5 μM sunitinib, 50 nM temsirolimus, 1 mg/ml bevacizumab, and combinations for 24 h. CXCL10 levels were measured by qRT-PCR, Levels of mRNAs were standardized to the expression of GAPDH. Mean ± SD, n = 3, *Indicates a significant difference from Control and single agent treatments, p < 0.05. (B) SK-LMS-1 cells (1 x 10⁷/mouse) were injected into the flanks of nude mice. When tumors reached approximately 150 mm³ in size, mice were randomized into groups and treated with 5 x 10⁸ TCID₅₀ Reolysin Q7D, 5 mg/kg temsirolimus QD, 40 mg/kg sunitinib QD, 10 mg/kg bevacizumab Q3D, or combinations as described in Materials and Methods. After 3 weeks of treatment, tumor volume was quantified. Mean ± SEM, n = 10. *Indicates a significant difference compared to vehicle or **either single agent treatment, p < 0.05. (C) Reolysin and drug combinations are well tolerated in mice. Animal body weight was determined at the end of the study (Day 21) to quantify drug-induced weight loss. Mean ± SD, n = 10.