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. 2017 Mar 28;6(6):1465–1476. doi: 10.1002/sctm.16-0128

Figure 2.

Figure 2

Generation of induced pluripotent stem cells (iPSCs) from urine sample of persons with Down syndrome. (A): Representative phase contrast images of transformation of urine‐derived T21 cells into T21‐iPSCs at 8, 25, 50 days after episomal vector transfection. Scale bar = 100 µm. (B): Representative immunofluorescence staining of pluripotent markers in CWRU1003i‐06T cell line colonies using antibodies against OCT4, SOX2, NANOG, TRA‐1‐60, and SSEA4. Cell nuclei were counterstained with DAPI. Scale bar = 50 µm. (C): Hematoxylin and Eosin staining of teratoma sections of iPSC clone CWRUi1002, P45, 59 days after injection. Scale bar, 100 μm. Abbreviation: DAPI, 4′,6‐diamidino‐2‐phenylindole, dihydrochloride.