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. 2017 Jul 29;6(9):1777–1785. doi: 10.1002/sctm.17-0002

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© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Changes of MSCs functional molecules and signaling pathways after stimulated by lupus peripheral blood mononuclear cells (PBMCs). Umbilical cord‐derived mesenchymal stem cells (UC MSCs) were cocultured with PBMCs from active lupus patients as well as healthy controls, and 48 hours later, the functional molecules including IL‐6 (A), CXCL12 (B), VEGF (C), and IL‐10 (D) from UC MSCs were assessed by real‐time polymerase chain reaction. The supernatant levels of CXCL12 (E) and VEGF (F) were analyzed by enzyme‐linked immunosorbent assay. Signaling pathways including Akt, IκB, Stat 5 (G), Smad1/5/8 and Erk1/2 (H) were assessed by Western blot. The changes of Akt, IκB, Stat 5 signaling pathways stimulated by recombinant IFN‐γ and TNF‐α were also assessed (I). *, p < .05; **, p < .01. Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; MSCs, mesenchymal stem cells; nPBMC, normal control peripheral blood mononuclear cells; sPBMC, SLE patient peripheral blood mononuclear cells; VEGF, vascular endothelial growth factor.