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. 2017 Jul 29;6(9):1777–1785. doi: 10.1002/sctm.17-0002

Table 1.

Primers for real‐time polymerase chain reaction

Gene Forward Reverse
TGF‐β1 5′‐AGCGACTCGCCAGAGTGGTTA‐3′ 5′‐GCAGTGTGTTATCCCTGCTGTCA‐3′
COX2 5′‐TGACCAGAGCAGGCAGATGAA‐3′ 5′‐CCACAGCATCGATGTCACCATAG‐3′
COX1 5′‐TGGTGCTGGCATGGATAGTA‐3′ 5′‐GGTTCTTGCTGTTCCTGCTC‐3′
IL‐6 5′‐AAGCCAGAGCTGTGCAGATGAGTA‐3′ 5′‐TGTCCTGCAGCCACTGGTTC‐3′
IDO1 5′‐GAATGGCACACGCTATGGAA‐3′ 5′‐CAGACTCTATGAGATCAGGCAGATG‐3′
IDO2 5′‐GGCTCTTGGGAAACTCCTTC‐3′ 5′‐TCAGGACATCACCAAAACCTT‐3′
IL‐10 5′‐CTCATGGCTTTGTAGATGCCT‐3′ 5′‐GCTGTCATCGATTTCTTCCC‐3′
CXCL‐12 5′‐TGGGCTCCTACTGTAAGGGTT‐3′ 5′‐TTGACCCGAAGCTAAAGTGG‐3′
VEGF 5′‐AGCTGCGCTGATAGACATCC‐3′ 5′‐CTACCTCCACCATGCCAAGT‐3′
GAPDH 5′‐GCACCGTCAAGGCTGAGAAC‐3′ 5′‐TGGTGAAGACGCCAGTGGA‐3′

Abbreviations: GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; VEGF, vascular endothelial growth factor.