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Quality control of the biosphincters. (A): Three dimensional engineered circular biosphincter: The engineered biosphincter has an internal lumen diameter of 7 mm. (B): The engineered IAS constructs were physiologically tested using an organ bath. When the biosphincters were hooked to the organ bath, they generated a spontaneous basal tone over time in the bath. Average basal tone generated by the biosphincters was 541 ± 13 µN. Abbreviation: IAS, internal anal sphincter.
